ABSTRACT
Objective To explore the clinical significance of detecting serum interleukin-10(IL-10) level and the proportion of myeloid-derived suppressor cells (MDSCs) in peripheral blood mononuclear cells (PBMCs) in children with bronchiolitis.Methods Fifty-one children with bronchiolitis less than 2 years old were randomly enrolled including 27 boys and 24 girls.They were divided into 2 groups:bronchiolitis group Ⅰ,25 children with atopic high risks were included in this group;bronchiolitis group Ⅱ,26 children without atopic high risks were included in this group.Children without infectious diseases such as hernia and renal calculus had been randomly enrolled as the control group (without atopic disease and atopic family disease) (n =45),including 25 boys and 20 girls.After taking 4 mL venous blood of patients in 3 groups,1 mL was used to test the serum proportion of MDSCs by flow cytometry,and the remaining blood was used to test the level of IL-10 in the serum by enzyme-linked immunosorbent assay.Results 1.The proportions of MDSCs in the PBMCs in bronchiolitis group Ⅰ [(3.17 ± 0.24) ％] and bronchiolitis group Ⅱ [(1.33 ±0.25) ％] were significantly higher than that of control group [(0.78 ± 0.25) ％] (all P ＜ 0.01),and the proportion of MDSCs in the PBMCs in bronchiolitis group Ⅰ was higher than that of bronchiolitis group Ⅱ (P ＜0.01).2.The serum levels of IL-10 in bronchiolitis bronchiolitis group Ⅰ [(31.88-± 3.91) ng/L] and bronchiolitis group Ⅱ [(23.85 ±4.10) ng/L] were significantly higher than that of control group [(13.63 ± 2.83) ng/L] (all P ＜0.01),and the levels of IL-10 in bronchiolitis group Ⅰ was higher than that of bronchiolitis group Ⅱ (P ＜ 0.01).3.There was a positive correlation between the proportion of MDSCs in the PBMCs and the serum levels of IL-10 in bronchiolitis group Ⅰ (r =0.717,P ＜ 0.01),but there was not any correlation between bronchiolitis group Ⅱ and control group (r =0.262,-0.102,all P ＞ 0.05).Conclusion MDSCs plays a crucial role by up-regulating the IL-10 level in the process of developing asthma from bronchiolitis.
ABSTRACT
<p><b>OBJECTIVE</b>To study changes to CD4(+)CD25(high+)CD127(low) regulatory T cells (Treg) in peripheral blood from children with bronchiolitis, and to explore its clinical significance.</p><p><b>METHODS</b>Thirty-one children with bronchiolitis and aged under two years were randomly enrolled as the bronchiolitis group, and 25 under two-year-olds with bronchopneumonia were randomly enrolled as the bronchopneumonia group. A further twenty-five children with non-infectious diseases such as hernia and renal calculus served as the control group. The level of CD4(+)CD25(high+)CD127(low) Treg in peripheral blood was measured by multi-color detection and multi-parameter flow cytometry.</p><p><b>RESULTS</b>The proportion of CD4(+)CD25(high+)CD127(low) Treg in peripheral blood in the bronchiolitis group (8.0%±2.1%) was significantly lower than in the bronchopneumonia (9.6%±2.6%; P<0.05) and control groups (11.3%±2.9%; P<0.05).</p><p><b>CONCLUSIONS</b>CD4(+)CD25(high+)CD127(low) Treg level in peripheral blood may be an index of immunological function in infants. A decreased level of CD4(+)CD25(high+)CD127(low) Treg in peripheral blood suggests that Treg cells may be involved in the pathogenesis and development of bronchiolitis.</p>